METHODS OF ISOLATING TUMOR-REACTIVE T CELL RECEPTORS FROM TUMOR OR PERIPHERAL BLOOD

摘要

The invention provides methods for generating or obtaining tumor-reactive T cells. Briefly, a high frequency CDR3β clonotype is used to select a full length β chain. The appropriate TCR α chain for pairing with this β chain is identified and polynucleotides encoding both chains of the TCR chains are constructed. These polynucleotides are introduced into PBMCs. The antitumor activity of TCR expressing transformants is determined and highly active transformants are considered for use in treating patients.