A25 bacteriophage lysin

摘要

The invention relates to the identification, sequencing, and isolation of an A25 bacteriophage lysin gene that expresses a protein involved in the lysis of bacterial cells during the phage life cycle. The invention further relates to methods for lysing certain bacteria using lysin, which are useful for example in a diagnostic procedure designed to detect these bacteria.

主权项

1. A method of detecting a Group A streptococcal analyte in a pathogenic streptococcal infection, comprising: a) providing a patient sample suspected of harboring Group A streptococci; b) contacting the sample with a PlyA polypeptide comprising an amino acid sequence with at least 85% sequence identity to SEQ ID NO: 2 and a purification tag, in an amount effective to lyse Group A streptococci if present in the sample; and c) detecting the presence or absence of a Group A streptococcal analyte present in the sample, wherein the presence of the Group A streptococcal analyte in the sample indicates the presence of a streptococcal infection in the subject.