METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION
摘要
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
申请公布号
TN2014000493(A1)
申请公布日期
2016.03.30
申请号
TN20140000493
申请日期
2014.11.25
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;CHARPENTIER, EMMANUELLE;UNIVERSITY OF VIENNA
发明人
CHARPENTIER, EMMANUELLE;DOUDNA, JENNIFER A;JINEK, MARTIN;CHYLINSKI, KRZYSZTOF;DOUDNA CATE, JAMES HARRISON;LIM, WENDELL;QI, LEI