Stabilization of alpha-amylases towards calcium depletion and acidic pH

摘要

The present invention relates to variants of a parent alpha-amylase, the variant having improved stability or activity at low calcium conditions or at low pH.

主权项

1. An isolated variant alpha-amylase, comprising two or more alterations at positions corresponding to positions 163, 188, 205, 208, and 209 of the polypeptide of SEQ ID NO: 2, wherein
(a) the variant has a sequence identity to SEQ ID NO: 8 of at least 90% and less than 100%; (b) each alteration is independently a substitution, deletion or insertion; and (c) the variant has alpha-amylase activity.