SEQUENCING STRATEGIES FOR GENOMIC REGIONS OF INTEREST

摘要

The disclosure relates to methods for determining the sequence of a genomic region of interest comprising a target nucleotide sequence comprising: providing a DNA comprising a genomic region of interest, fragmenting the DNA, separating the DNA fragments, fragmenting and ligating the DNA fragments to provide for ligated fragments of the DNA fragments (or ligated DNA subfragments), and determining at least part of the sequences of at least part of the ligated DNA subfragments that comprise the target nucleotide sequence.

主权项

1. A method for determining the sequence of a genomic region of interest comprising a target nucleotide sequence, the method comprising the steps of:
providing a DNA comprising the genomic region of interest; fragmenting the DNA to provide DNA fragments; separating the fragmented DNA; fragmenting the separated DNA fragments to provide DNA subfragments and ligating the DNA subfragments; determining at least part of the sequences of at least part of the ligated DNA subfragments that comprise the target nucleotide sequence; and using the determined sequences to build a contig of the genomic region of interest.