GENETIC CONTROL METHOD AND COMPOSITIONS AGAINST INSECT PESTS IN COTTON PLANTS BY THE SILENCING OF GENES OF THE LACCASE FAMILY

摘要

The present invention relates to the control of infestation of pests by inhibiting or reducing the expression of genes of the family of chitin synthase. The invention further provides methods and compositions for controlling pests by feeding them with one or more double-strand RNA molecules provides by the present invention. The invention further describes a method of obtaining transgenic plants that express double-strand RNA molecules. The present invention is preferably used for cotton-plants.

主权项

1. An isolated nucleic acid molecule, characterized by being selected from the group consisting of:
a. an isolated nucleic acid molecule comprising a nucleic acid sequence substantially similar to the sequences selected from the group of: SEQ ID No 1, SEQ ID No 2; b. an isolated nucleic acid that hybridizes a nucleic acid sequence indicated by the sequences selected from the group of SEQ ID No 1, SEQ ID No 2, under 5×SSC wash conditions, formamide at 50% and 42° C.; for 10 min; c. a fragment of at least 19 continuous nucleotides of a nucleic acid sequence selected from the group of SEQ ID No 1, SEQ ID No 2, wherein the ingestion, by coleopterous pests, of the double-strand ribonucleotide sequence, comprising at least one complementary strand of said fragment, inhibits or reduces the proliferation of said pest; and d. a complement of the sequence of (a), (b) or (c).