Method for producing 5′-guanylic acid

摘要

5′-guanylic acid (GMP) is produced efficiently by allowing a microorganism to react with xanthylic acid (XMP), wherein said microorganism is able to convert xanthylic acid into 5′-guanylic acid and has been modified so that the nagD gene does not function normally and 5′-guanylic acid synthetase activity is enhanced.

主权项

1. A method for producing 5′-guanylic acid comprising:
A) reacting a microorganism with xanthylic acid to produce 5′-guanylic acid, and B) collecting 5′-guanylic acid;wherein said microorganism is able to convert xanthylic acid into 5′-guanylic acid, and has been modified so that:
a) a nagD gene does not function normally, and b) 5′-guanylic acid synthetase activity is enhanced as compared to a non-modified microorganism by increasing expression of a guaA gene in said microorganism,wherein said guaA gene encodes a protein comprising an amino acid sequence having an identity of not less than 95% to the entire amino acid sequence of SEQ ID NO: 2, 4, 6, or 8, andwherein said protein has 5′-guanylic acid synthetase activity, and wherein said microorganism is bacteria selected from the group consisting of Enterobacteriaceae bacteria, Bacillus bacteria, and coryneform bacteria.