The invention relates to a method for culturing mammalian stem cells, in particular embryonic stem cells comprising the following steps: a) providing a perfused bioreactor (1) comprising a cell culture chamber (2); b) placing said mammalian stem cells within said culture chamber (2); c) providing a perfusion loop which provides fresh medium to said perfused bioreactor and removes used medium from said perfused bioreactor; d) providing a dialysis loop which comprises a reservoir of medium (3) and dialysis chamber (4); wherein the dialysis loop provides fresh medium to the perfusion loop through the dialysis chamber (4). The invention also relates to a device for culturing mammalian stem cells according to the invention.
申请公布号
WO2009016078(A3)
申请公布日期
2009.04.09
申请号
WO2008EP59644
申请日期
2008.07.23
申请人
INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE);CAILLERET, MICHEL;COME, JULIEN;PESCHANSKI, MARC