YOPM AS DELIVERY VEHICLE FOR CARGO MOLECULES AND AS BIOLOGICAL THERAPEUTIC FOR IMMUNOMODULATION OF THE INFLAMMATORY REACTIONS

摘要

The present invention relates to the use of Yersinia outer protein M (YopM), a YopM fragment, or a YopM variant, which is capable of autopenetrating the cell membrane and of integrating into the cell cytosol without the requirement of additional factors for delivering a cargo molecule across the membrane to the cytosol of a cell. The present invention also relates to a pharmaceutical composition comprising YopM, a YopM fragment, or a YopM variant being capable of autopenetrating the cell membrane and of integrating into the cell cytosol without the requirement of additional factors for the regulation of inflammatory reactions of the immune system and the treatment of diseases caused by autoimmunity of the host. The present invention further relates to a YopM fragment or variant, which is capable of autopenetrating the cell membrane and of integrating into the cell cytosol without the requirements of additional factors as well as such proteins or YopM linked to a cargo molecule.

主权项

1. A pharmaceutical composition, comprising
(i) an isolated YopM; (ii) an isolated YopM fragment, comprising at least one alpha helix of YopM capable of mediating autopenetration; or (iii) an isolated YopM variant, characterized in that said variant
(a) consists of an amino acid sequence that is at least 80% identical to any of SEQ ID NOs: 1-8 or at least 80% identical to a fragment of any of SEQ ID NOs: 1-8; and(b) comprises at least one alpha helix of YopM capable of mediating autopenetration; in the absence of Yersinia bacteria; wherein said YopM, YopM fragment or YopM variant is capable of autopenetrating the cell membrane and integrating into the cell cytosol of a eukaryotic cell without the assistance of a type III secretion system.