METHODS AND COMPOSITION FOR SECRETION OF HETEROLOGOUS POLYPEPTIDES

摘要

The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns signal sequences for the secretion of heterologous polypeptide from bacteria. The invention also concerns recombinant polypeptides and uses thereof.

主权项

1. A method of making an a heterologous polypeptide, said method comprising culturing a host cell comprising polynucleotide comprising a variant translation initiation region (TIR) comprising nucleic acid variants of a PhoA, MalE, DsbA or STII secretion signal region operably linked to a polynucleotide encoding the heterologous polypeptide, so that the polynucleotide is expressed, whereby upon expression of the heterologous protein in a host cell, the heterologous polypeptide is folded to form a biologically active heterologous polypeptide.