发明名称 |
High-yield peptide antibiotics producing strain, preparation method and use thereof |
摘要 |
High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization. |
申请公布号 |
US8911968(B2) |
申请公布日期 |
2014.12.16 |
申请号 |
US201113994633 |
申请日期 |
2011.12.15 |
申请人 |
Shanghai Techwell Biopharmaceutical Co., Ltd. |
发明人 |
Chen Yi;Liu Shidong;Zhang Zhaoli;Wang Chunxia;Kang Jing;Ji Xiaoming |
分类号 |
C12P21/04;C07K7/54;C12R1/645;C12N15/01;C12N1/14;C07K7/56 |
主分类号 |
C12P21/04 |
代理机构 |
McDermott Will & Emery LLP |
代理人 |
McDermott Will & Emery LLP ;Jordan Carey C. |
主权项 |
1. A mutagenized strain of Coleophoma empetri, deposited in China General Microbiological Culture Collection Center with the Accession No. CGMCC 4129. |
地址 |
CN |