MICROSCOPE FOR WIDEFIELD MICROSCOPY

摘要

A microscope for microscopy having a detection beam path and an illumination beam path. The microscope includes a filter wheel device, which is arranged in the detection beam path and/or illumination beam path and has a filter wheel, wherein the filter wheel is mounted to rotate about an axis, and wherein the filter wheel is divided into segments. Filters forming a first part-circle are arranged in a first part of the segments, such that said filters are consecutively introduced into the detection beam path or illumination beam path when the filter wheel is rotated. A camera records images at a predefined frequency. The filter wheel device includes a motor-driven shaft that rotates the filter wheel at a predefined rotation frequency. The microscope also comprises a control system for synchronising the image-recording frequency and rotation frequency based on the filters arranged on the filter wheel.

主权项

1. A microscope for widefield microscopy with a detection beam path and an illumination beam path, comprising:
a filter wheel device including, at least one filter wheel mounted on a motor-driven shaft so as to be rotatable about an axis at a specified rotational frequency, the at least one filter wheel arranged in the detection beam path, or in the. detection beam path and the illumination beam path, the at least one filter wheel being divided into a plurality of segments, a first plurality of filters forming a first divided circle being arranged in at least a first part the segments, so that each filter in the first plurality of filters is successively introduced into the detection beam path or the illumination beam path when the filter wheel is rotated, and a second plurality or filters' forming at least one more divided circle, arranged in at least the first part of the segments, so that the segments of the first part contain at least two filters arranged in succession in a radial direction, the filter wheel device further comprising means for deflecting light reflected by a first filter in a segment of the first part of segments onto a second filter in the same segment; at least one camera with a planar detector in the detection beam path, designed to record images at a specified frame rate; and a control system for synchronizing the frame rate and the rotational frequency depending, on the filters arranged on the at least one filter wheel.