Sensitive and rapid determination of antimicrobial susceptibility

摘要

The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

主权项

1. A method for the identification of microorganisms in a sample comprising:
contacting a sample with a device comprising a chamber and at least one capture surface; capturing microorganisms on the capture surface, wherein an individual microorganism binds to the capture surface at a spatially discrete location; introducing a first indicator to the device, wherein the first indicator is configured to bind to a first type of microorganism; and detecting the presence of the first indicator.