One dose vaccination against <i>Mycoplasma </i>infections of pigs

摘要

The present invention provides a one phase, aqueous vaccine composition for immunizing an animal against infection by Mycoplasma hyopneumoniae, comprising: an immunizing amount of a Mycoplasma hyopneumoniae bacterin, an acrylic acid polymer in the concentration range between 0.8 and 1.2 mg/ml, and a pharmaceutically acceptable carrier, and substantially no oil. It is especially useful for immunizing a pig against infection by Mycoplasma hyopneumoniae for at least 20 weeks after a single administration, which effective immunity is reached within 4 weeks after vaccination.

主权项

1. A method of preparing a one phase aqueous composition comprising a Mycoplasma hyopneumoniae bacterin, an acrylic acid polymer in a concentration range between 0.8 and 1.2 mg/ml, a pharmaceutically acceptable carrier, and substantially no oil;
comprising:
a. cultivating a M. hyopneumoniae whole cell culture such that the Mycoplasma hyopneumoniae bacterin has an antigen amount of at least 3 log 10 per ml;b. inactivating the M. hyopneumoniae whole cell culture;c. isolating an immunizing amount of a Mycoplasma hyopneumoniae bacterin;d. admixing an acrylic acid polymer in the concentration range between 0.8 and 1.2 mg/ml; ande. admixing a pharmaceutically acceptable carrier.