Tomographic phase microscopy

摘要

The present invention relates to systems and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle. A preferred embodiment provides tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure and the quantitative characterization of specimen-induced aberrations in high-resolution microscopy with multiple applications in tissue light scattering.

主权项

1. An interferometric method for measuring refractive index of a medium comprising:
scanning light along a first scanning light path through a medium at a plurality of angles, a medium holder being stationary relative to a detector during scanning of the light path; combining the light transmitted through the medium with a temporally modulated reference light; detecting the combined light with the detector to generate measured data; and processing the measured data with a data processor to determine a distribution of a refractive index of the medium.