Methods and Compositions for Plant Pest Control

摘要

The present invention comprises methods and compositions for controlling nematode parasitism in host plant. The present invention comprises novel polynucleotides and polypeptides encoded by such polynucleotides comprising one or more nucleic acid sequences disclosed herein having a nucleotide sequence comprising any one of SEQ ID NOs: 1-142, a fragment or variant thereof, or a complement thereof, or a polypeptide sequence comprising any one of SEQ ID NOs: 143-159, a fragment or variant thereof.

主权项

1. A nucleic acid construct, comprising, a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence comprising any one of SEQ ID NOs: 1-142, a fragment or variant thereof, or a complement thereof; (b) a nucleotide sequence comprising at least 90% sequence identity to any one of SEQ ID NOs: 1-142, a fragment or variant thereof, or a complement thereof, wherein said nucleotide sequence encodes a silencing element having nematocidal activity against a nematode plant pest; (c) a nucleotide sequence comprising at least 19 consecutive nucleotides of any one of SEQ ID NOs: 1-142, a fragment or variant thereof, or a complement thereof, wherein said nucleotide sequence encodes a silencing element having nematocidal activity against a nematode plant pest; (d) a nucleotide sequence that hybridizes under stringent conditions to the full length complement of the nucleotide sequence of a), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said nucleotide sequence encodes a silencing element having nematocidal activity against a nematode plant pest; and, (e) a nucleotide sequence encoding a polypeptide sequence comprising any one of SEQ ID NOs: 143-159, a fragment or variant thereof.