| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for preparing a pancreatic islet that produces glucose-induced insulin and has a three-dimensional structure including plural functional endocrine cells, as with the pancreatic islet in vivo, by differentiating pluripotent stem cells.SOLUTION: The invention provides a method for preparing pancreatic Langerhans islet from a pluripotent stem cell, the method comprising the steps of: differentiating a pluripotent stem cell to a certain stage of the pancreatic cell lineage; dispersing the differentiated cells to single cells; and culturing the dispersed single cells using cells differentiated from the pluripotent stem cell to a certain stage of the pancreatic cell lineage as the feeder cells. |
