| 摘要 |
FIELD: chemistry.SUBSTANCE: invention represents a photosensitiser (PS) for photodynamic therapy (PDT) of cancer and other neoplasms of different genesis, as well as fluorescent diagnostics, where PS contains a trismeglumin salt of chlorine e6, and as a cryostabiliser - methyl glucamine in a ratio of 1:0.1-0.2 wt.p. The invention also relates to a method of obtaining the photosensitiser for PDT, which includes dilution of methylpheophorbide a in acetone, processing the obtained solution with aqueous alkali (NaOH or KOH) with following neutralisation of a reaction mixture by diluted hydrochloric acid to pH 4.5-5.0, separation of the precipitated sediment of chlorine e6 with following washing with distilled water, processing the sediment with a water solution and lyophilisation, which is characterised by the fact, that processing of methylpheophorbide a in acetone with aqueous alkali is carried out at a temperature of 40-50°C, separation of chlorine e6 sediment is realised by filtering through a layer of celite 545, and chlorine e6 is extracted from celite by a water solution of meglumine until the concentration of chlorine e6 salt, corresponding to optic density D=225-235/1 ml, is achieved.EFFECT: obtaining a lyophilised drug form PS without ballast substances, which has an increased storage stability, which results in simplification and optimisation of chlorine e6 solution with a high degree of purity.2 cl, 2 ex, 2 dwg |
