| 摘要 |
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date H(10) International Publication Number 3 February 2011 (03.02.2011) PCT W O 2011/014838 Al (51) International Patent Classification: Embarcadero Center, Eighth Floor, San Francisco, Cali C12N 5/00 (2006.01) C12N 9/64 (2006.01) fornia 94111-3834 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2010/044020 kind of national protection available): AE, AG, AL, AM, .) . AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (22) International Fing Date: Jul 2010 (30.07.2010) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (26) Publication Language: English ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (30) Priority Data: NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 61/230,477 31 July 2009 (31.07.2009) US SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicants (for all designated States except US): BAX TER INTERNATIONAL INC. [US/US]; One Baxter (84) Designated States (unless otherwise indicated, for every Parkway, Deerfield, Illinois 60015 (US). BAXTER kind of regional protection available): ARIPO (BW, GH, HEALTHCARE S.A. [CH/CH]; Thurgauerstrasse 130, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, CH-8152 Glattpark (Opfikon) (CH). ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (72) Inventors; and EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (75) Inventors/Applicants (for US only): GRILLBERGER, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, Leopold [AT/AT]; Dresdnerstrasse 51/8, A-1200 Vienna SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (AT). SPENGER, Alexandra [AT/AT]; Hippgasse 36 / GW, ML, MR, NE, SN, TD, TG). 39, A-1160 Vienna (AT). HASSLACHER, Meinhard Published: [AT/AT]; Vorgartenstrasse 221/1/7, A-1020 Vienna (AT). GRILLBERGER, Rana [LB/AT]; Dresdnerstrasse 51/8, - with international search report (Art. 21 (3)) A-1200 Vienna (AT). REITER, Manfred [AT/AT]; Ge- - before the expiration of the time limit for amending the border Lang Gasse 11/17, A-1 150 Vienna (AT). claims and to be republished in the event of receipt of (74) Agents: FLANAGAN, Lisa Dornbach et al.; amendments (Rule 48.2(h)) TOWNSEND and TOWNSEND and CREW LLP, Two (54) Title: CELL CULTURE MEDIUM FOR ADAMTS PROTEIN EXPRESSION Surface Plot of spe. activity normalized vs Temp; pH r (57) Abstract: The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as CADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. i some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein. |
