CONTROLLED INHIBITION AND RE-ACTIVATION OF DNA POLYMERASES BY CLEAVABLE OLIGONUCLEOTIDE INHIBITORS

摘要

A hot start enzyme composition is described that includes a hot start nuclease, a nucleic acid polymerase, and a substantially double-stranded oligonucleotide that inhibits the catalytic activity of the nucleic acid polymerase at temperatures lower than the melting temperature of the oligonucleotide.