OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE BINDING, DETECTION, DIFFERENTIATION, ISOLATION AND SEQUENCING OF HERPES SIMPLEX VIRUS

摘要

Described herein are primers and probes useful for the binding, detecting, differentiating, isolating, and sequencing of HSV -1 and/or HSV-2 viruses. One embodiment is directed to an isolated nucleic acid sequence comprising a sequence selected from the group consisting of: SEQ ID NOS: 1-19. One embodiment is directed to a method of hybridizing one or more isolated nucleic acid sequences comprising a sequence selected from the group consisting of: SEQ ID NOS: 1-19 to an HSV -1 and/or HSV-2 sequence, comprising contacting one or more isolated nucleic acid sequences to a sample comprising the HSV -1 and/or HSV-2 sequence under conditions suitable for hybridization. In a particular embodiment, the sequence is a genomic sequence, a naturally occurring plasmid, a naturally occurring transposable element, a template sequence or a sequence derived from an artificial construct.