| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a rapid, reliable and simple method for the detection or the species identification of an acid-fast bacterium which is important on clinical diagnosis. <P>SOLUTION: This method comprises carrying out a melting curve analysis with a probe whose target nucleic acid is a region having high bacteria species specificity in dnaJ1 gene in an acid-fast bacterium. The method comprises hybridizing a primer or an elongation product of the primer with an oligonucleotide probe used for detection and containing a nucleic acid sequence comprising at least ten continuous bases among specific nucleic acid sequences to form a complex, and then discriminating the formed complex. <P>COPYRIGHT: (C)2009,JPO&INPIT |
