发明名称 |
METHOD FOR PURIFYING HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR FROM RECOMBINANT E. COLI |
摘要 |
<p>The present invention provides a method for purifying a large amount of human granulocyte-colony stimulating factors (hG-CSFs) from a recombinant E. coli with high yield and purity. According to the method of the present invention, human granulocyte-colony stimulating factor, identical to the native form expressed in the human body, can be easily purified with high yield and purity without an additional activation process. In particular, according to the purification method of the present invention, hG-CSF variants expressed in E. coli are efficiently removed to obtain physiologically active hG-CSFs with high purity.</p> |
申请公布号 |
EP2632944(A2) |
申请公布日期 |
2013.09.04 |
申请号 |
EP20110836621 |
申请日期 |
2011.10.26 |
申请人 |
HANMI SCIENCE CO., LTD. |
发明人 |
CHOI, SUNG CHUL;KIM, JIN KI;OH, YOUNG HAK;LEE, JONG SOO |
分类号 |
C07K14/53;C07K1/16;C12N15/27;C12N15/70 |
主分类号 |
C07K14/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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