ANTIBIOTICS-INDEPENDENT VECTOR FOR CONSTANT HIGH-EXPRESSION AND METHOD FOR GENE EXPRESSION USING THE SAME

摘要

PURPOSE: Provided are an antibiotics-independent vector for constant high-expression and a method for gene expression using the same. The antibiotics-independent vector can be applied to high expression of useful DNAs and proteins, and production of recombinant proteins. CONSTITUTION: The antibiotics-independent plasmid vector is constructed by using a major amino acid composing a cell wall and a gene associated with D-glutamic acid synthase, as an auxotrophic maker, and it contains DNA or its fragment which is represented by the SEQ ID NO:1. The antibiotics-independent plasmid vector expresses a foreign gene in the absence of antibiotics, and shows D-glutamic acid synthesis activity in a bacterium. The foreign gene is characteristically selected from the group consisting of nucleic acids coding proteins, anti-sense RNA, and ribozyme. A transformant including the expression vector is cultured without adding antibiotics to its culture medium in order to give proteins.