发明名称 |
COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION |
摘要 |
<p>The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.</p> |
申请公布号 |
CA2801564(A1) |
申请公布日期 |
2011.12.15 |
申请号 |
CA20112801564 |
申请日期 |
2011.06.09 |
申请人 |
SYNGENTA PARTICIPATIONS AG |
发明人 |
BASU, SHIB SANKAR;DANG, KRISTEN KAMERATH;AZHAKANANDAM, KASIMALAI;MCNEILL, THOMAS Z.;GUO, SHENG |
分类号 |
C40B30/02;C12N15/00;C12P21/00;C12Q1/68 |
主分类号 |
C40B30/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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