发明名称 METHOD OF OBTAINING BIOLOGICALLY ACTIVE SUBSTANCE FROM EMBRIONIC-EGG MASS FOR PREPARATION OF ANTI-BURN PLATE AND ANTI-FIRE PLATE ON ITS BASE
摘要 FIELD: medicine. ^ SUBSTANCE: non-infected fresh hen eggs are selected with their further incubating for 9 days. On 5, 6, 7 days hen egg is radiated for 30 s at frequency of modulation 9 Hz and radiation voltage 9 mW with further cooling of eggs for 6-7 days at temperature 2-4C and homogenisation of eggs. Obtained homogenate is subjected to processing by ultrasound in ultrasonic dispersant. To homogenate, processed with ultrasound, added is mixture, containing 0.1% solution of sodium benzoate and 0.1% of solution of potassium sorbate in equal parts, in weight ratio 1:1 respectively with further ultramicrofiltration through filters with 0.2 mcm diameter, obtaining biologically active substance for embryonic-egg mass. Homogeneous mass is prepared in the following way. Medical gelatin is covered with decoction, containing calendula decoction and chamomile decoction in weight ratio 1:1, keep at temperature 20-25C for 55-60 minutes with further placement of decoction with gelatin on water bath at temperature 65-70C until complete dissolusion of gelatin is obtained. Into obtained decoction with gelatin added are amikacin, furacilline, anesthesin, amaranth oil, glycerol and brilliant green with further mixing in homogeniser for 4-5 min obtaining homogeneous mass. To obtained homogeneous mass biologically active substance from embryonic-egg mass is added in specified ratio and mixed in homogeniser for 5-7 min. Obtained mass is poured into special vessels and are placed in thermostat for 3.5-4 hours at temperature 37-39C with further cooling for 1-2 days until plates solidify in form of plates. ^ EFFECT: inventions make it possible for injuries to regenerate quickly, increase immune reactivity of the organism and make it possible to simplify method of obtaining anti-burn plate. ^ 2 cl, 6 dwg, 2 tbl, 5 ex
申请公布号 RU2433171(C1) 申请公布日期 2011.11.10
申请号 RU20100124737 申请日期 2010.06.16
申请人 TIMCHENKO LJUDMILA DMITRIEVNA;RZHEPAKOVSKIJ IGOR' VLADIMIROVICH 发明人 TIMCHENKO LJUDMILA DMITRIEVNA;RZHEPAKOVSKIJ IGOR' VLADIMIROVICH;VAKULIN VALERIJ NIKOLAEVICH;ZATONA ELENA GENNAD'EVNA;POKHODENKO MARIJA VITAL'EVNA
分类号 A61K9/00;A61K35/66;C12N1/38 主分类号 A61K9/00
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