COMPOSITION FOR SEPARATELY DETECTING MYCOBACTERIUM TUBERCULOSIS COMPLEX AND THE MYCOBACTERIUM GENUS, AND METHOD FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND THE MYCOBACTERIA GENUS BY A REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION USIN

摘要

The present invention relates to a composition for separately detecting mycobacterium tuberculosis and the mycobacterium genus, comprising: (i) a primer and/or a probe that targets IS6110 which is a mycobacterium-tuberculosis-specific gene; (ii) a primer and/or a probe that targets an inter-transcriptional spacer (ITS) which is a mycobacterium-genus-specific gene; and optionally (iii) a primer and/or a probe which is an internal control and which targets a plant-derived gene. When a real-time multiplex polymerase chain reaction is performed using the composition of the present invention, not only mycobacterium tuberculosis but also the mycobacterium genus can be detected through the single reaction, which thus enables clinical diagnosis to be performed in a quick and easy manner with high reliability.