MONOCLONAL ANTIBODY FOR DISCRIMINATING AND DETECTING ABNORMAL PRION CAUSING BOVINE SPONGIFORM ENCEPHALOPATHY

摘要

PURPOSE: A monoclonal antibody for specifically detecting abnormal prion of spongiform encephalopathy is provided. CONSTITUTION: A fusion cell line of deposit number KCLRF-BP-00213 produces monoclonal antibody for detecting abnormal prion of spongiform encephalopathy. A method for preparing fusion cell line comprises: a step of inoculating a peptide of sequence number 1 to a knock-out mouse; a step of isolating B cells from the mouse; and a step of fusing the B cells and mouse myeloma-derived cells. The abnormal prion of spongiform encephalopathy is detected by western blotting using the monoclonal antibody.