Method for detecting abnormal spots of nucleic acid microarray

摘要

A method carries out a nucleic acid analysis using a nucleic acid microarray. A probe nucleic acid including a probe sequence (a') complementary to a target sequence (a) and a sequence (b') which is different from the probe sequence (a') is immobilized on the nucleic acid microarray. The method includes hybridizing the nucleic acid microarray and a labeled abnormality detecting nucleic acid (B) containing a sequence (b) which can be bound to the sequence (b'), obtaining a labeled amount value (Fc1) of the labeled abnormality detecting nucleic acid (B) from a spot (X1), and determining, based on the measured labeled amount value (Fc1), as to whether or not the spot (X1) is an abnormal spot unsuitable for detecting the target nucleic acid.