摘要 |
<p>A solid-phase substrate detection system amplification medium comprising a solvent, thickener, and a signal developing reagent for causing signal precursor molecules bound to a target on a solid-phase substrate to generate a signal indicating the presence and/or quantity of the target where the medium hinders diffusion of the signal. The thickener may comprise gel forming polymers, polysaccharides, proteins, natural gums, surfactants, agar, agarose, and gelatin. The signal developing reagent may be a base, an enzyme or an enzyme substrate, and may comprise oxidoreductases, transferases, hydrolases, or esterases. Also specified is a method comprising applying the medium to a solid substrate. The medium may be applied as a thin film by spraying, dipping, dropping, or immersing the substrate in the medium. Also specified is a test device comprising means for transporting fluid sample from an application site to a test site, a conjugate comprising a specific binder for the target and a detectable signal precursor material, and the medium.</p> |