METHOD FOR ISOLATING AND PRESERVING GAMETOPHYTES OF KJELLANIELLA CRASSIFOLIA

摘要

A method for mass-producing gametophytes of Kjellmaniella crassifolia is provided to be usefully used for ecological restoring of the endangered Kjellmaniella crassifolia through mass-proliferation of the gametophytes and contribute to income increase of a fisherman by diversifying species of culture fishes. A method for mass-producing gametophytes of Kjellmaniella crassifolia comprises the steps of: (a) after cutting apothecia of Kjellmaniella crassifolia, removing impurities therefrom with sterile seawater; (b) inducing the apothecia to release zoospores; (c) germinating the released zoospores in a liquid culture medium or a solid culture medium to form gametophytes; (d) after aging the formed gametophytes in a solid medium, isolating them into female and male gametophytes; (e) homogenizing the isolated female and male gametophytes using a homogenizer and then cutting them to have a size of 10-50 micrometers; and (f) culturing the cut female gametophytes in a liquid medium at a temperature of 10-24 deg.C with the intensity of radiation of 15-70 muE/m^2xs^-1 and culturing the cut male gametophytes in a liquid medium at a temperature of 1-10 deg.C with the intensity of radiation of 40-80 muE/m^2xs^-1. A method for preserving the zoospores of the Kjellmaniella crassifolia comprises the steps of: (a) after cutting the apothecia of the Kjellmaniella crassifolia, removing impurities therefrom with sterile seawater; (b) inducing the apothecia to release the zoospores; and (c) preserving the released zoospores in a solid medium. Further, the liquid culture medium is Provasoli's enriched seawater media.