METHOD OF IDENTIFICATION BETWEEN HANWOO AND FOREIGN BEEF MEATS

摘要

A method for identifying Korean native cattle and imported beef is provided to accurately identify the Korean native cattle derived from Bos taurus and the imported beef derived from Bos indicus by using structural differences of an RFLP genotype of a sex-determining region Y(SRY) gene. The method comprises the steps of: (a) collecting DNA from Korean native cattle and imported beef respectively; (b) respectively combining a forward primer having a nucleotide sequence of 5'-TTAGAACGCTTACACCGCATATTACTTC-3' and a backward primer having a nucleotide sequence of 5'-TAGTAAAATTGAGATAAAGAGCGCCTTTGTTAG-3' of an SRY gene with each of the collected DNA; (c) amplifying the combined product through PCR in which a nucleotide sequence portion of 1003-1803 in a Bos taurus DNA(registration number : AB039748) and a nucleotide sequence portion of 273-1073 of a Bos indicus DNA(registration number : AY079145) are amplified; and (d) after cutting the amplified product with an MseI restriction enzyme, analyzing polymorphism thereof to divide genotypes of the Korean native cattle and the imported beef.