| 摘要 |
PROBLEM TO BE SOLVED: To prevent the occurrence of positive errors in measuring creatine and creatinine for diagnoses of muscle diseases and renal diseases by reducing reactivity to proline by modifying a protein having sarcosine oxidase activity using a protein engineering method. SOLUTION: Reactivity of a natural sarcosine oxidase to proline is reduced by a method, etc., in which the 345th phenyl alanine in the amino acid sequence of the formula as a protein having sarcosine oxidase activity, is substituted with alanine, glycine, valine or isoleucine. |
