| 摘要 |
A microorganism for production of L-threonine and L-tryptophan is produced by using a vector by transformation, transduction and/or conjugation, and comprises polynucleotides, alleles, open reading frames, and/or promotor, where the polynucleotide/alleles copy number is increased to 1 by integration of gene/allele into the chromosome of the microorganism and is obtained by an extra chromosomal replication vector and the concentration of the polynucleotide/activity of the product (e.g. protein) is strengthened by increasing to 10%, is new. A microorganism for production of L-threonine and L-tryptophan is produced by using a vector by transformation, transduction and/or conjugation, and comprises polynucleotides, alleles, open reading frames, and/or promotor, where the polynucleotide/alleles copy number is increased to 1 by integration of gene/allele into the chromosome of the microorganism and is obtained by an extra chromosomal replication vector and the concentration of the polynucleotide/activity of the product (e.g. protein) is strengthened by increasing to 10%. The coded amino acid sequence is 80% identical to the amino acid, e.g. Met-Lys-Thr-Arg-Asp-Ser-Gln-Ser-Ser-Asp (SEQ ID NO: 2). An independent claim is also included for the production of L-amino acids by fermentation of recombination microorganisms of the Enterobacteriaceae family. |
