发明名称 |
Method for extracting DNA from organisms |
摘要 |
A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.
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申请公布号 |
US2006188967(A1) |
申请公布日期 |
2006.08.24 |
申请号 |
US20060338364 |
申请日期 |
2006.01.24 |
申请人 |
NALIN RENAUD;ROBE PATRICK;VAN VAN T |
发明人 |
NALIN RENAUD;ROBE PATRICK;VAN VAN T. |
分类号 |
C12P19/34;C12N15/10;G01N27/447 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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