PRIMER FOR DETECTION OF CYTOCHROME P450 HYDROXYLASE SPECIFIC TO POLYENE

摘要

The present invention relates to a primer for detection of cytochrome P450 hydroxylase specific to polyene and a method for detecting a strain of bacteria which produces polyene, by using the primer. More particularly, the present invention is directed to a forward degenerate primer of following sequence (I) and a reverse degenerate primer of following sequence (II), which are complementary to conserved region which resides in the polyene-specific CYP (cytochrome P450 hydroxylase) encoding sequences;(I)5'-TGGATCGGCGACGACCGSVYCGT-3' and (II) 5'-CCGWASAGSAYSCCGTCGTACTT-3', wherein S indicates Guanine or Cytosine, V indicates Adenine, Guanine or Cytosine, Y indicates Thymine or Cytosine and W indicates Thymine or Adenine.