CHROMATOGRAPHY SYSTEM, METHOD AND SOFTWARE FOR THE SEPARATION OF BIOMOLECULES

摘要

The present invention relates to methods, systems and software for optimising the separation of a sample of biomolecules eluted from a chromatography column in which the concentration of a second component added to an elution buffer is varied in order to form an elution buffer solution of varying second component concentration. The concentration of the elution buffer solution is held at a current value or increased at a slower rate when a hold gradient event occurs and the varying of the concentration of the elution buffer solution is restarted or increased at a higher rate after a restart gradient event occurs. A hold gradient event occurs when a detector signal relating to the amount of biomolecules in the elution buffer solution rises past a first threshold and a restart gradient event occurs when the signal falls past a second threshold.