| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for producing an adeno-associated virus (AAV) vector useful for human gene therapy in a sufficient amount. <P>SOLUTION: Disclosed is a method for generating high titers of recombinant AAV vectors which are available for delivery systems, wherein the method comprises a process for incubating cells under a condition enabling replication and packaging of AAV, wherein the cells comprises (a) a vector providing at least one intact copy of a stably integrated recombinant AAV vector in the cells, wherein the AAV vector comprises AAV inverted terminal repeat (ITR) regions and a transcription promoter operably linked to a target polynucleotide, and wherein the expression of the rep gene is limiting in the cells; and (b) a plasmid providing an AAV packaging plasmid that allows expression of the product of the rep gene, wherein in the plasmid the rep gene is operably linked to a heterologous promoter, and recombinant AAV vectors are generated thereby. <P>COPYRIGHT: (C)2005,JPO&NCIPI</p> |
