发明名称 |
Generation of transgenic mice by transgene-mediated rescue of spermatogenesis |
摘要 |
The present invention provides transgenic mice and nucleic acid constructs and methods for creating them. The transgenic mice are generated preferably by electroporation-mediated transfection of a nucleic acid construct into the testes of a sterile spermatogenesis-deficient male mouse. The construct includes DNA encoding a spermatogenesis rescue factor resulting in rescue of spermatogenesis while contemporaneously transferring a nucleic acid of interest for expression in the transgenic mouse so that progeny sired by the transgenic mouse also harbor the transgene. Certain embodiments of the invention also utilize (a) the salmonid derived Sleeping Beauty transposition system to augment genomic integration of the transgene and/or (b) the Cre/loxP recombination system for excision from sired progeny of the DNA responsible for spermatogenesis rescue.
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申请公布号 |
US2005071895(A1) |
申请公布日期 |
2005.03.31 |
申请号 |
US20040498113 |
申请日期 |
2004.06.09 |
申请人 |
ZHANG NIAN |
发明人 |
ZHANG NIAN |
分类号 |
A01K67/027;C12N15/85;C12N15/873;(IPC1-7):A01K67/027 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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