ETHANOL-INDUCIBLE EXPRESSION VECTOR TO OBTAIN INTEREST GENE FROM PLANTS AT CERTAIN CULTURING PERIOD OR STAGE BY USING ETHANOL

摘要

PURPOSE: An ethanol-inducible expression vector is provided, thereby easily obtaining an interest gene from plants at a certain culturing period or stage by using ethanol as an inducing chemical which can be sprayed in a large scale due to its strong volatility. CONSTITUTION: The ethanol-inducible expression vector comprises the nucleotide sequence(SEQ ID NO:1) encoding the ADR1 site which is a transcription factor of Saccharomyces cerevisiae; the nucleotide sequence(SEQ ID NO:2) encoding a promoter of ADH2(alcohol dehydratase 2); and an interest gene operably linked to the promoter, wherein the ADR1 site essentially comprises the nucleotide sequence(SEQ ID NO:3) encoding the TADI site which is the zinc finger site; the ADH2 promoter essentially comprises the nucleotide sequence(SEQ ID NO:4) encoding the UAS site(200bp DNAs); the ADH2 promoter is ADH2-35S fusion promoter having the nucleotide sequence of SEQ ID NO:15; and the alcohol is ethanol.