| 摘要 |
The invention relates to a method for dosing telluric acids most often present in a residual form, in a preparation originating from a culture of Gram+ bacteria. This method firstly requires a hydrolysis controlled by hydrofluoric acid at a temperature lower than or equal to 40 DEG C in order to liberate the specific oligosaccharides from the telluric acids. The dosing of the specific oligosaccharides can then be carried out by employing diverse techniques, particularly by high-performance chromatography coupled with a pulsed field amperometric detection (HPAEC-PAD). The inventive method can, in particular, serve to dose residual quantities of telluric acids present in preparations containing capsular polysaccharides of Streptococcus pneumoniae that can be used as vaccines. |
