| 摘要 |
The present invention provides a recombinant plasmid, comprising (a) a ubiquitous promoter, (b) one fluorescent gene, said gene being operably linked to and inserted downstream of said ubiquitous promoter, (c) a skin-specific or muscle-specific promoter, and (d) another fluorescent gene, said gene being operably linked to and inserted downstream of said skin-specific or muscle-specific promoter, wherein the ubiquitous promoter and the skin-specific or muscle-specific promoter have the adverse directional property and the ubiquitous promoter and the skin-specific or muscle-specific promoter are located upstream of said fluorescent gene and said another fluorescent gene respectively so as to have the directional property which permits transcription of said genes. Also provided a host cell, a method of producing a transgenic fish and transgenic fish harboring the plasmid of the invention.
|
