| 摘要 |
A method is provided for the integration of foreign genetic elements into a bacterial chromosome without the need of a cloning step. The method relies on the presence of the lambda-Red recombinase system in a recombination proficient bacterial host. At least two linear recombination elements or constructs are co-transformed into the recombination proficient host which are assembled in the correction orientation in the bacterial chromosome by homologous recombination. Selectable markers used for the selection of transformants are later excised by the action of a site-specific recombination.
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