SEQUENTIAL HYBRIDIZATION OF FUNGAL CELL DNA AND METHOD FOR THE DETECTION OF FUNGAL CELLS IN CLINICAL MATERIAL

摘要

In order to detect fungal cells in clinical material, fungal DNA is extracte d from whole blood, after which the extracted fungal DNA is analysed. On the basis of this analyse a fungal infection can be indicate d. For further diagnosis the fungal species is subsequently determined from the extracted fungal DNA. The process for the extraction of fungal DNA from whole blood encompasses the isolation of predominantly intact fungal cells from whole blood and the extraction of DNA from the isolated fungal cells. In order to analyse the fungal DNA at least one segment of said fungal DNA is amplified, whereupon the amplification products are, if required, analysed. 1n order to provide further identification of the fungal species nucleotide sequence segments characteristic of the species of fungus are determined.