发明名称 |
Method for the detection of minority genomes in virus quasispecies using dna microchips |
摘要 |
A method for the detection of minority genomes in virus quasispecies using DNA microchips. The method makes it possible to detect minority genomes, more particularly minority memory genomes, in a nucleic acids population of a virus quasispecie, which are present in a population of less than 50%, containing at least one mutation relative to the majority genomes of the quasispecie. The method involves the following steps: a) extracting the nucleic acid of the virus quasispecie form a sample susceptible of containing the virus quasispecie; b) amplifying at least one fragment of the nucleic acid of the virus quasispecie, and c) detecting and analyzing the existence of minority genomes using DNA microchip-base techniques. The method can be used in genetic diagnosis of viral diseases.
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申请公布号 |
US2003180717(A1) |
申请公布日期 |
2003.09.25 |
申请号 |
US20030258765 |
申请日期 |
2003.05.05 |
申请人 |
ESTEBAN ARMANDO ARIAS;BARANOWSKI ERIC;LLORENTE CARLOS BRIONES;SOLANS ESTEBAN DOMINGO;ESCARMIS HOMS CHRISTINA;GOMEZ CASTILLA JORDI;MARTIN RUIZ-JARABO CARMEN;PARRO GARCIA VICTOR |
发明人 |
ESTEBAN ARMANDO ARIAS;BARANOWSKI ERIC;LLORENTE CARLOS BRIONES;SOLANS ESTEBAN DOMINGO;ESCARMIS HOMS CHRISTINA;GOMEZ CASTILLA JORDI;MARTIN RUIZ-JARABO CARMEN;PARRO GARCIA VICTOR |
分类号 |
C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70 |
主分类号 |
C12Q1/68 |
代理机构 |
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