| 摘要 |
<p>A flow cytometer, and at least one reconstruction cylinder are positioned around a capillary tube (2). A photon source (25) and a photon sensor (26) work together with a pulse height analyzer (27) to provide a first trigger point (28) for the beginning of an object or cell (1), and a second trigger point (29) for the end of the cell (1). The trigger signal (28, 29) is received by the reconstruction cylinder (20). The reconstruction cylinder (20) includes optical point sources (21) having a selectable emission wavelength, disposed in a geometric pattern around the cylinder perpendicular to and concentric with the capillary tube axis (9) that facilitate the acquisition of transmitted, attenuated projection images of the flowing cells (1). The sensors (23) also collect projections of fluorescence emitted from tagged molecular probes associated with nuclear and/or cytoplasmic structures or cell membranes. The projections are algorithmically processed to provide three dimensional information about the cells (1) and their disease state.</p> |
