发明名称 |
Counter selection strategy for gram-negative bacteria |
摘要 |
A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention. |
申请公布号 |
AU8354701(A) |
申请公布日期 |
2002.02.18 |
申请号 |
AU20010083547 |
申请日期 |
2001.08.07 |
申请人 |
THE BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA |
发明人 |
STEPHEN K. FARRAND;PAUL E. STASWICK;THOMAS E. CLEMENTE |
分类号 |
C12N1/21;C12N15/65;C12N15/74;C12N15/82 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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