The invention concerns a method for screening a repertoire of polypeptides to identify one or more members thereof which interact with one or more target molecules, comprising: a) immobilising the target molecule(s) on a support; b) arranging a plurality of nucleic acid molecules encoding the repertoire of polypeptides in an array; c) juxtaposing the target molecule(s) and the arrayed nucleic acid molecules; d) expressing the arrayed nucleic acid molecules to produce the polypeptides such that said polypeptides come into contact with the target molecule(s) on the support and a subset of the polypeptides interacts with the target molecules; and e) detecting the interaction of the polypeptides with the target molecules on the support. The invention also provides a high density antibody array consisting of thousands of different polypeptide features, spatially arranged on a solid support for screening against different target ligands.
申请公布号
WO0140803(A1)
申请公布日期
2001.06.07
申请号
WO2000GB04638
申请日期
2000.12.04
申请人
DIVERSYS LIMITED;HOLT, LUCY, JESSICA;DE WILDT, RUDOLF, MARIA, THEODORA;TOMLINSON, IAN
发明人
HOLT, LUCY, JESSICA;DE WILDT, RUDOLF, MARIA, THEODORA;TOMLINSON, IAN