APPARATUS AND METHODS FOR PRODUCING AND USING HIGH-DENSITY CELLS AND PRODUCTS THEREFROM

摘要

Disclosed and claimed is apparatus and methods for the growth of cells to hi gh density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cells at high cell density with wild type a nd recombinant baculoviruses to produce baculovirus and DNA or gene or expessio n products. The apparatus comprises three interconnected modules, the cell culturing loop (100), the media replenishment loop (200) and the hollow fibe r dialysis device (300). Cells from the bioreactor (110) are transported throu gh the lumen space (310) of hollow fiber dialysis device and media from the med ia replenishment loop (200) is pumped through the extra-lumen space (320). Prio r to re-entering the bioreactor the cells with media have oxygen added via the oxygenator (151).