| 摘要 |
In separation of 3 components A, B and C having a C>B>A relation in respect of affinity for packing with a chromatographic separator comprising a circulation system formed by linking packing bed units 1 to 4 in endless series, the following Operations &lang&2&rang&->&lang&4&rang&->&lang&1&rang&->&lang&4&rang&->&lang&3&rang& are performed in Step (1) as Stage 1, and the following Operations &lang&2&rang&->&lang&4&rang&-> &lang&3&rang& are repeatedly performed 3 times in subsequent Stages 2 to 4 in Step (2), provided that the fluid feed and withdrawal positions are respectively displaced one by one in the downstream direction of the circulation system every time after completion of each stage; &lang&1&rang&: shutting off the circulation system, and withdrawing the whole of a component B fraction on the upstream side of the shutoff position while feeding a starting fluid material f to a packing bed unit on the downstream side of the shutoff position and feeding eluent D to the circulation system; &lang&2&rang&: withdrawing part of a component A fraction while feeding eluent D to the circulation system; &lang&3&rang&: withdrawing part of a component C fraction while feeding eluent D to the circulation system; and &lang&4&rang&: circulating the internal fluid through the system with neither fluid feed to nor fluid withdrawal from the circulation system. <IMAGE> |
